We made a re-expanded version for the database of thermal death of microorganisms, ThermoKill Database (TKDB) R8110. The database was built using data adopted and collected from research papers published between 1981 and 2010 in 25 major selected academic journals. The database includes thermal death data such as 20,467 D values and 3,272 z values along with predicted D values at reference temperatures, other microbial information, environmental conditions and bibliographic data for a total of 70 items. Representative statistical distribution data obtained from the novel database were demonstrated and discussed the significance of its practical use in food industry.