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标题：Propensity to high-fat diet-induced obesity in mice is associated with the indigenous opportunistic bacteria on the interior of Peyer’s patches
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作者：Yi Qiao ; Jin Sun ; Zhenxing Xie 等
期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：2014
卷号：55
期号：2
页码：120-128
DOI：10.3164/jcbn.14-38
出版社：The Society for Free Radical Research Japan
摘要：Indigenous opportunistic bacteria on the interior of the Peyer’s patches play a key role in the development of the mucosal immune, but their population composition has been ignored. The present study was conducted to test the hypothesis that the changes in the composition of indigenous opportunistic bacteria in the Peyer’s patches are associated with obesity. C57BL/6J-male mice had been fed either a control diet or a high-fat diet. After 25 weeks, mice in high-fat diet exhibit either an obesity-prone (OP) or an obesity-resistant (OR) phenotype. Control diet group (CT) and OR group had a significant larger bacteria diversity than that in the OP group. Allobaculum and Lactobacillus were significantly decreased in high-fat diet induced OP mice compared with CT and OR mice, whereas Rhizobium and Lactococcus was significantly increased. The result of quantitative real-time PCR was consistent with that of 454 pyrosequencing. Significant correlations between mRNA expression of inflammation marks and the top 5 abundance genera bacteria on the interior of Peyer’s patches were observed by Pearson’s correlation analysis. Taken together, the indigenous opportunistic bacteria on the interior of Peyer’s patches plays a major role in the development of inflammation for an occurrence of obesity.
关键词：indigenous opportunistic bacteria;inflammation;obesity;Peyer’s patches;454 pyrosequencing