文章基本信息

标题：Large-scale Preparation of 1,2-β-Glucan Using 1,2-β-Oligoglucan Phosphorylase
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作者：Koichi Abe ; Masahiro Nakajima ; Motomitsu Kitaoka 等
期刊名称：Journal of Applied Glycoscience
印刷版ISSN：1344-7882
电子版ISSN：1880-7291
出版年度：2015
卷号：62
期号：2
页码：47-52
DOI：10.5458/jag.jag.JAG-2014_011
出版社：The Japanese Society of Applied Glycoscience
摘要：1,2-β-Glucan was produced enzymatically from 1.0 M sucrose and 0.5 M glucose by the combination of sucrose phosphorylase and 1,2-β-oligoglucan phosphorylase in the presence of 100 mM inorganic phosphate. Accumulation of 1,2-β-glucan in 2 L of the reaction mixture reached over 800 mM (glucose equivalent). Sucrose, glucose and fructose were removed after the reaction by yeast treatment. 1,2-β-Glucan was precipitated with ethanol to obtain 167 g of 1,2-β-glucan from 1 L of the reaction mixture.