文章基本信息

标题：Glassin, a histidine-rich protein from the siliceous skeletal system of the marine sponge Euplectella, directs silica polycondensation
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作者：Katsuhiko Shimizu ; Taro Amano ; Md. Rezaul Bari 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2015
卷号：112
期号：37
页码：11449-11454
DOI：10.1073/pnas.1506968112
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：SignificanceHexactinellid sponges of the genus Euplectella produce highly ordered and mechanically robust skeletal systems of amorphous hydrated silica. The high damage tolerance of their constituent skeletal elements and the environmentally benign conditions under which these sponges form have prompted additional investigations into the characterization of the proteins driving the synthesis of these materials. In the present report, we describe a previously unidentified protein, named "glassin," extracted from the demineralized skeletal elements of Euplectella. Glassin is a histidine-, aspartic acid-, threonine-, and proline-rich protein and directs silica polycondensation at neutral pH and room temperature. The hexactinellids are a diverse group of predominantly deep sea sponges that synthesize elaborate fibrous skeletal systems of amorphous hydrated silica. As a representative example, members of the genus Euplectella have proved to be useful model systems for investigating structure-function relationships in these hierarchically ordered siliceous network-like composites. Despite recent advances in understanding the mechanistic origins of damage tolerance in these complex skeletal systems, the details of their synthesis have remained largely unexplored. Here, we describe a previously unidentified protein, named "glassin," the main constituent in the water-soluble fraction of the demineralized skeletal elements of Euplectella. When combined with silicic acid solutions, glassin rapidly accelerates silica polycondensation over a pH range of 6-8. Glassin is characterized by high histidine content, and cDNA sequence analysis reveals that glassin shares no significant similarity with any other known proteins. The deduced amino acid sequence reveals that glassin consists of two similar histidine-rich domains and a connecting domain. Each of the histidine-rich domains is composed of three segments: an amino-terminal histidine and aspartic acid-rich sequence, a proline-rich sequence in the middle, and a histidine and threonine-rich sequence at the carboxyl terminus. Histidine always forms HX or HHX repeats, in which most of X positions are occupied by glycine, aspartic acid, or threonine. Recombinant glassin reproduces the silica precipitation activity observed in the native proteins. The highly modular composition of glassin, composed of imidazole, acidic, and hydroxyl residues, favors silica polycondensation and provides insights into the molecular mechanisms of skeletal formation in hexactinellid sponges.
关键词：fusion materials ; Porifera ; biomineral ; silicon dioxide ; organic–inorganic composite