Stress is everywhere in modern life. Increasingly more problems are emerging because of stress, which cause lots of economic losses. Based on the theoretical analysis, with first-hand data collection and using multiple regression models, this study explored the relationship between neuroticism, stressor and stress response and figured out interactive effect of neuroticism and stressor on stress response. We draw on the following conclusions: (1) The interaction term of stressor (work) and neuroticism as well as three stressors (health, family and social) are predictors of physiological stress response; (2) The interaction term of stressor (work) and neuroticism, two stressors (health and social) are predictors of physiological stress response; (3) The interaction term of stressor (health) and neuroticism as well as two stressors (social and family) are predictors of behavioral stress response; (4) The interaction term of stressor (health) and neuroticism, as well as three stressors (social, family and work) are predictors of total stress response.