Small round structured virus (SRSV) genes were detected from 14 of 1, 366 fecal samples which were collected from healthy food handlers working at school lunch facilitiesusing reverse transcription-polymerase chain reaction (RT-PCR) during one year between June 1997 and May 1998. The SRSV genes were detected from two persons after twoweeks but not after four weeks; thus, the excretion of SRSV genes was believed to continue for at least two weeks at least. Analysis of the SRSV gene sequences confirmed that these strains belonged to two different types in genogroup II of Norwalk-like viruses.