When we performed melezitose assimilation test in industrial yeasts, most sake yeasts failed to assimilate melezitose, while the other industrial yeasts could. We therefore tried to elucidate differences for melezitose assimilation among industrial yeasts. Results showed industrial yeasts had no melezitose resolving power on the cell surface, but had this in the cell. Also, the sake yeasts had melezitose resolving power in the cell. There were remarkable differences among industrial yeasts when we examined transportation ability in a cell. In addition, both melezitose transportation ability into cells and resolving power in cells were increased by using maltose as a carbon source.