The turbid material was separated by centrifuging directly or after treating with Spitase F This purified material contained 35-65% of protein, 45-65% of carbohydrate, and a little phosphate. Glycine, alanine, valine, leucine, serine, threonine, aspartic acid, and glutamic acid were identified by two--dimensional paper chromatography in acid hydrolyzate, but basic amino acids, methionine, tyrosine, and proline could not be detected. Mannose, glucose, galactose and isomaltose were identified by multiple paper chromatography in weak acid hydrolyzate. These sugars were also contained in the hydrolyzate of yeast and Aspergillus oryzae . Therefore, this turbid material is presumed to have a close connection with the autolysis of these microorganisms. The dimension of the particles of this turbid material was about 100 Å, but when precipitated with Spitase F, other particles of 500-1300 Å were observed by electron -microscopy.