1. By using 5 species of coniferous, 10 species of deciduous and 3 species of bamboo wood meals, the Maule's and Cl₂-Na₂So₃ reactions were examinde, and the absorption spectra of their colors were measured. In the case of coniferous wood, the absorption peak near 390mμ was appeared in both reactions, whereas two absorption peaks near 390mμ and 510mμ were appeared with deciduous wood and the absorption spectrum of bamboo was similar to that of deciduous wood. 2. From the above results, the authors assumed that the absorption peak near 390mμ was re-sponsible for the guaiacylpropane component, whereas the other absorption peak near 510mμ was caused by the syringylpropane component of the lignin, and then both reactions were examined with some guaiacyl, syringyl and p-hydroxyphenxl compounds. Consequently, on the basis of absorption spectrum and stability of color, syringaldehyde, syringic acid and gal-lic acid gave the color characteristics of deci-duous wood-their absorption peaks were near 510 mμ-whereas guaiacyl compounds such as vanillin, ferulicacid and coniferylalcohol gave only absorption peak near 39mμ. 3. About 1 kg of each wood meal was chlorinated, or treated with 1% KMnO₄ and then with 12% HCI respectively and thereafter each lignin preparation which gave positive color reaction was extracted by cold ethanol and was purified. Both lignin preparations were similar at fol-lowing points: a) The contents of OCH₃, and Cl₂. b) Solubility and ultraviolet absorption spectrum. C) Both vanillin and syringaldehyde were found at the alkaline nitrobenzene oxidation. However, As a difference between both pre-paration was found on the stability of color which appeared for alkali, it was suggested that there were some different point of structure in detail. 4. From the above results it was suggested that both reactions were due to the existence of syringyl nucleus contained in the lignin, and then the building unit of the lignin could be classified by these reactions. Accordingly, the lignin formation was examined by these reactions on the incubation experiment of embryonic root tip of kidney bean and bamboo-shoot tissue in some phenylpropane substances. 5. As SIEGEL's experimental results showed, the formation of the substance which gave positive color reaction was accelerated by the addition of H₂O₂ and phenylpropane substance, and the formation of this substance was inhibited by some inhibitors of heavy metal containing en-zyme, such as NaN₃, NH₂OH, CH₂ICOOH, H₂S and NO, as well as KCN and auxin. Then, it was sugested that the peroxidase was responsible to the lignin formation. 6. No syringaldehyde was found in alkaline nitro-benzene oxidation of incubated sample with guaiacylpropane substances, whereas in the case of incubated sample with syringylpropane sub-stances, both Mäule's and Cl₂-Na₂SO₃ reactions became poitive and syringaldehyde was found in alkaline nitrobenzene oxidation. Accordingly, it was suggested that the formation of syringyl nucleus was occurred at the earlier stage than the formation of phenylpropane nucleus. 7. Euenol and dihydroconiferylalcohol were not transformed to the lignin by the isolated peroxi-dase or phenoloxidase, whereas FREUDENBERG was found that the coniferylalcohol was trans-formed to the lignin by the isolated phenoloxi-dase, or peroxidase. Then it was suggested that the eugenol and dihydroconiferolalcohol were oxidized to the coniferylalcohol type and then it was transformed to the lignin by the phenoloxidase and or peroxidase action.