The aim of this study was to establish a simple and novel equation to determine the 50%VO₂max/wt (ml/kg/min) predicted by the one-point method (1 PM50%VO₂max/wt) using data from daily exercise aimed at health promotion, and to elucidate the relationship between this 1 PM50% VO₂max/wt and coronary risk factors (CRFs). Data from 632 female subjects (aged 41±13 years) were studied. The 1 PM50%VO₂max/wt was calculated from paired values of work rate and heart rate during exercise on a stationary bicycle ergometer, and the age-predicted heart rate at 50%VO₂max (=138-age/2). As CRFs, percentage body fat, systolic blood pressure, diastolic blood pressure, total cholesterol, triglyceride, high-density lipoprotein cholesterol, fasting blood sugar and HbA1c were measured. Logistic regression analysis was carried out for statistical analysis. Our findings confirmed that the odds ratio of having abnormal values for CRFs was lower at a higher 1 PM50%VO₂max/wt ( p <0.001), indicating that data obtained from daily training aimed at health promotion are very valuable for calculating 1 PM50%VO₂max/wt for assessment of health status.