The purpose of the present study was to examine the influence of tendon elasticity, muscle strength and muscle activities on the amount of mechanical work enhancement associated with a counter movement. Twenty-one athletes performed a unilateral maximal jump using only the ankle joint with (CMJ) and without (no-CMJ) a counter movement on a sledge apparatus. Mechanical work done by the ankle joint was calculated from the ground reaction force and ankle joint kinematic parameters, and the difference between CMJ and no-CMJ conditions (ΔWork) was determined. During the exercise, electromyographic (EMG) activities were recorded from the triceps surae muscles. The maximal isometric plantar flexion torque and Achilles tendon stiffness were also determined using a torque meter and ultrasonogram. No significant correlation was found between ΔWork and either tendon stiffness or the maximal torque. In addition, neither the difference between CMJ and no-CMJ in iEMG nor mEMG was correlated with ΔWork. On the other hand, ΔWork was significantly correlated with the integrated EMG during the braking phase (r=0.52, p<0.05) and both the integrated (r=0.55, p<0.01) and mean (r=0.53, p<0.05) EMG during the push-off phase of CMJ. These results suggest that individual differences in ΔWork are influenced not by differences in the mechanical properties of the muscle-tendon unit, but by the individuality of muscle activities during CMJ.