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标题：Identification by Genetic Examination of Three Cestodes Isolated from Patients: Diphyllobothrium nihonkaiense, Larval Spirometra erinaceieuropaei and Taenia saginata
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作者：Niichiro ABE ; Isao KIMATA ; Shigehiko UNI 等
期刊名称：生活衛生
印刷版ISSN：0582-4176
电子版ISSN：1883-6631
出版年度：2009
卷号：53
期号：3
页码：169-176
DOI：10.11468/seikatsueisei.53.169
出版社：Osaka Urban Living and Health Association
摘要：Four cestode samples collected from four patients were identified by homology searching of the partial mitochondrial cytochrome oxidase subunit 1 gene (COX1) sequences. The COX1 region was amplified by PCR using a pair of JB3 and JB4.5 or DBCOI-1 and DBCOI-2 primers for the Pseudophyllidea cestode and by multiplex PCR for the Cyclophyllidea cestode. The COX1 sequences of two samples speculated to be Diphyllobothrium nihonkaiense and D. latum were morphologically identical and homology searching showed that the sequences of the two samples had 99.6% to 100% identity with those of D. nihonkaiense . The two samples were therefore identified as D. nihonkaiense . Similarly, a sequence from a larva isolated from the great omentum of one patient showed 98% to 100% identity with that of Spirometra erinaceieuropaei . This sample was therefore identified as larval S. erinaceieuropaei . The remaining sample tested positive for Taenia saginata in multiplex PCR and the sequence from the diagnostic fragment had 99.7% to 100% identity with that of T. saginata . This sample was therefore identified as T. saginata . The present findings highlight the usefulness of genetic examination for the diagnosis of cestodiasis.
关键词：Genetic examination;Diphyllobothrium nihonkaiense;Spirometra erinaceieuropaei;Taenia saginata;PCR