文章基本信息

标题：Molecular Characterization of Entamoeba histolytica Isolates in Osaka City
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作者：Niichiro ABE ; Noriko MITSUNO ; Eiji IKEDA 等
期刊名称：生活衛生
印刷版ISSN：0582-4176
电子版ISSN：1883-6631
出版年度：2007
卷号：51
期号：6
页码：399-401
DOI：10.11468/seikatsueisei.51.399
出版社：Osaka Urban Living and Health Association
摘要：The protozoan parasite, Entamoeba histolytica , is a causative agent of human amebiasis. Recent molecular studies show the genetic diversity among the isolates, and therefore identification of the isolates at the genotype level is important for control of the E. histolytica infection and for understanding the population structure of E. histolytica genotypes. In the present study, we identified the genotypes of the twenty-two isolates from patients in Osaka City by sequencing analysis of three polymorphic loci. Four genotypes (B, E, D, G) were found in locus 1-2, eight (A5, A5v, A6, A7, Cv, A6/Cv, A10/A5, A10/A6) in locus 5-6 and four (A, B, C, D, F) in chitinase. The following genotype at each locus was dominant type: genotype E in locus 1-2 (68%), A10/A5 in locus 5-6 (33%), B in chitinase (58%). Moreover, the isolates in Osaka City examined were classified into nine types (I to IX) based on the combination of genotypes. The present study showed the genetic polymorphism among the isolates in Osaka City and the possible presence of isolates peculiar to Osaka City.
关键词：Entamoeba histolytica;genetic polymorphism;genotypes;sequencing analysis