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标题：Identification of Anisakinae Larvae by Diagnostic PCR
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作者：Niichiro ABE ; Kinpei YAGI
期刊名称：生活衛生
印刷版ISSN：0582-4176
电子版ISSN：1883-6631
出版年度：2005
卷号：49
期号：3
页码：168-171
DOI：10.11468/seikatsueisei.49.168
出版社：Osaka Urban Living and Health Association
摘要：Anisakids are parasitic nematodes infecting fish and mammals. It is well recognized that human infection with this parasite, called anisakiasis, occurs by ingestion of raw or undercooked fish. In Japan, there are estimated to be over 1,000 cases of this infection annually. In 1999, “The Food Sanitation Law Enforcement Regulation” was partly amended, and anisakiasis was newly added to the causative agents of food poisoning. Identification of anisakid larvae has been performed by light microscopy, but it is impossible to accurately identify the worms morphologically when only small portions of the worm are available. In the present study, we showed the usefulness of PCR-based methods for identification of anisakid species using several species of anisakid larvae from Pacific cod. The larvae identified morphologically as Anisakis simplex or Contracaecum osculatum were found also to be positive for PCR amplification with only species-specific primers. In addition, the larvae of Pseudoterranova decipiens , frequently found in anisakiasis in Japan, were positive for amplification with only the P. decipiens -specific primers designed for the study. PCR using species-specific primers is thus concluded to be a useful tool for identification of anisakid larvae when morphological identification is impossible.
关键词：Anisakis;Contracaecum;Identification;PCR;Pseudoterranova