The purpose of the study was to assess whether the point of deflection from linearity of heart rate (HRD) could be used as an alternative method to determine the ventilatory threshold (VT) in Indian (Bengali) boys that represents the determination of the anaerobic threshold (AT), and also to standardize an exercise test to be effective in eliciting AT in Indian (Bengali) boys by using HRD. Twenty six (26) boys with a mean age of 12.8 (±1.18) years performed a graded maximal exercise test on a treadmill to determine peak VO₂, HRD and VT. The mean peak VO₂, weight related peak VO₂, peak pulmonary ventilation, and peak heart rate of the boys were found to be 1.75 l/min, 47.1 ml/kg/min, 66.9 l/min and 200.2 beats/min respectively. There were no significant differences between mean VO₂, weight related VO₂, pulmonary ventilation (VE), heart rate and respiratory exchange ratio (RER) that were measured at VT and HRD. The mean VO₂ measured at VT and HRD was found to be 1.46 and 1.45 l/min, which were about 84% and 83% of their respective peak values. Linear regression analysis revealed a correlation of 0.94 ( p <0.01) between VO₂ measured at VT and VO₂ measured at HRD, so the present study indicates that the point of deflection from linearity of heart rate (HRD) may be an accurate predictor of VT in most but not all boys.