The purpose of the present study was to determine the degree of fitting an approximation equation for oxygen uptake (Vo₂) in decrement-load exercise (DLE). Work rate was started from 120 watts and was decreased by a rate of 15 watts per min. The initial work rate of DLE corresponded to 72±10% of the work rate at anaerobic threshold determined in incremental-load exercise (ILE). Vo₂ in DLE increased rapidly, reached a peak, and decreased linearly until the end of the exercise. Vo₂ in DLE was higher than that in ILE at the same work rate except in the early periods in ILE and DLE. This difference ranged from 300 to 400 ml/min. This difference is a result of repayment of oxygen debt in DLE and from the oxygen deficit induced by the delay of response of Vo₂ in ILE. As work rate in DLE can be obtained by the difference between work rates in constant-load exercise (CLE) and ILE, we postulated that the approximation equation for Vo₂ kinetics in DLE could be expressed by a combination of approximation equations in CLE and in ILE. When time delay was taken into consideration in this equation, the fitting of data obtained by using the equation was better than that of data obtained by using the equation without a parameter of time delay. The degree of fitting ranged from 94 to 98% (r²). Thus, it seems that Vo₂ including oxygen debt in DLE can be approximated by the equation used in this study.