文章基本信息

标题：Ultraviolet Action Spectrum for Cell Killing of Primary Porcine Lens Epithelial Cells
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作者：Tsutomu Okuno ; Takako Nakanishi- Ueda ; Toshihiko Ueda 等
期刊名称：Journal of Occupational Health
印刷版ISSN：1341-9145
电子版ISSN：1348-9585
出版年度：2012
卷号：54
期号：3
页码：181-186
DOI：10.1539/joh.11-0215-OA
出版社：Japan Society for Occupational Health
摘要：Objectives: The aim of this study was to determine ultraviolet (UV) action spectra for cell killing of primary porcine lens epithelial cells (LECs) that can be used to establish guidelines for evaluation of the hazard of cataract due to UV exposure in the workplace. Methods: Primary porcine LECs were exposed to different doses (radiant exposure) of UV at 17 different wavelengths from 235 nm to 311 nm. At 2 days after exposure, cell viability was assessed by measuring crystal violet staining of the cells and lactate dehydrogenase release into the culture medium. The exposure dose required to kill 50% of cells (LD₅₀) was determined from the dose-effect relationship obtained at each wavelength and was used to construct action spectra. Results: The action spectra had a broad minimum in the approximate range of 250–280 nm, indicating that UV is most hazardous to porcine LECs within this wavelength range. The spectra rose steeply at both longer and shorter wavelengths. These action spectra are consistent with the in vivo action spectra for opacities in the rabbit lens and for light scattering in the rat lens, taking the transmittance of the ocular media into account. Conclusions: These results will help to determine a UV hazard function for cataract formation, which can be used to draft guidelines for evaluation of the hazard of cataract due to UV exposure in the workplace.
关键词：Action spectrum;Cataract;Cell killing;Hazard evaluation;Lens epithelial cell;Ultraviolet radiation