文章基本信息

标题：Characteristic Analysis of Peripheral Blood Mononuclear Cell Apoptosis in Coke Oven Workers
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作者：Hong Mei Zhang ; Ji Sheng Nie ; Xin Li 等
期刊名称：Journal of Occupational Health
印刷版ISSN：1341-9145
电子版ISSN：1348-9585
出版年度：2012
卷号：54
期号：1
页码：44-50
DOI：10.1539/joh.11-0155-OA
出版社：Japan Society for Occupational Health
摘要：Objectives: The aim of the present study was to determine the peripheral blood mononuclear cell (PBMC) apoptosis in coke oven workers so that we can take effective measures to protect coke oven workers. Methods: The subjects, 129 coke oven workers and 37 warehouse workers (controls), were investigated using a questionnaire to collect information about their age, working years, smoking and drinking habits, vocational history and other general information. The coke oven workers were divided into the oven-bottom group (34), oven-side group (48) and oven-top group (47) according to their working sites and environmental monitoring data. The concentration of benzo[a]pyrene (B[a]P) and the subjects’ urinary 1-hydroxypyrene (1-OH-Py) levels were determined by HPLC. Additionally, the PBMCs were separated from blood samples, and the early and late apoptosis rates were determined by flow cytometry. Results: The airborne B[a]P concentrations were 19.5 ± 13.2, 185.9 ± 38.6 and 1,623.5 ± 435.8 ng/m³ at the bottom, side and top of the oven, respectively, and were higher than in the controls’ workplaces 10.2 ± 7.6 ng/m³. Urinary 1-OH-Py, indicating the B[a]P’s internal exposure level, was significantly higher in the exposed groups than in the controls ( p Conclusions: PBMC apoptosis in the coke oven workers was associated with the 1-OH-Py level, coke operation years and years of alcohol consumption and may be induced by B[a]P.
关键词：Alcohol;B[a]P;Coke operation years;Immunity;1-OH-Py;PBMC apoptosis