文章基本信息

标题：Detection of Mutant p53 Protein in Workers Occupationally Exposed to Benzidine
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作者：Cui-Qin Xiang ; Chun-Lin Shen ; Zi-Rong Wu 等
期刊名称：Journal of Occupational Health
印刷版ISSN：1341-9145
电子版ISSN：1348-9585
出版年度：2007
卷号：49
期号：4
页码：279-284
DOI：10.1539/joh.49.279
出版社：Japan Society for Occupational Health
摘要：To investigate the expression of mutant p53 protein in workers occupationally exposed to benzidine, we detected mutant p53 protein by immuno-PCR assay in the serum of 331 benzidine-exposed healthy workers, while we classified exfoliated urothelial cells in urine samples with Papanicoloau's grading (PG). The Papanicoloau's grading classified exfoliated urothelial cells of the subjects from grade I (normal cells) to grade III (suspicious malignant cells). The subjects were also divided into high, medium and low exposure groups according to the exposure intensity index. The results revealed that mutant p53 protein in the medium and high exposure groups were significantly higher than the in low exposure group ( p <0.05), and in PG II and III were significantly higher than in the PG I ( p <0.05). There was no significant differences among Papanicoloau's gradings strata in the low exposure group on the incidence and quantity of mutant p53 protein. In the medium and high exposure groups, the incidence and/or quantity of mutant p53 protein in the stratum of PG II and/or III were significantly higher than that of PG I ( p <0.05). Detection of mutant p53 protein in conjunction with benzidine exposure level and Papanicoloau's gradings of exfoliated urothelial cells could provide more information to help us elevate surveillance efficiency and diagnose bladder cancer in the early period.
关键词：Benzidine;Exposure intensity index;mutant p53 protein;Exfoliated urothelial cells;Papanicoloau's grading