文章基本信息

标题：Differential Expression of EC-SOD, Mn-SOD and CuZn-SOD in Rat Lung Exposed to Crystalline Silica
本地全文：下载
作者：Heungnam Kim ; Yasuo Morimoto ; Akira Ogami 等
期刊名称：Journal of Occupational Health
印刷版ISSN：1341-9145
电子版ISSN：1348-9585
出版年度：2007
卷号：49
期号：3
页码：242-248
DOI：10.1539/joh.49.242
出版社：Japan Society for Occupational Health
摘要：Superoxide dismutases (SODs) are antioxidant enzymes that catalyze the dismutation of superoxide into hydrogen peroxide. There are 3 kinds of isozymes: extracellular superoxide dismutase (EC-SOD), manganese-containing superoxide dismutase (Mn-SOD) and copper- and zinc-containing superoxide dismutase (CuZn-SOD). To examine the expression of SOD isozymes in lungs injured by crystalline silica, we intratracheally instilled male Wistar rats with 2 mg (8 mg/kg) of crystalline silica and investigated the mRNA, protein level and distribution of SOD isozymes in the rat lungs using RT-PCR, western blot analysis and immunostaining, respectively at from 3 d to 180 d of recovery following the exposure. EC-SOD mRNA levels significantly increased from 3 d to 90 d and the EC-SOD protein level was significantly higher after 90 and 180 d recovery in the crystalline silica exposed groups than in the control groups. Mn-SOD increased in silica treated rat lungs at both mRNA and protein levels, peaking at 30 d post-exposure. CuZn-SOD mRNA levels were decreased at 3, 7 and 30 d, and CuZn-SOD protein levels were also significantly lower than the control group at 90 and 180 d recovery. There was prominent EC-SOD immunostaining mainly in the plasma and alveolar macrophages and strong Mn-SOD staining in alveolar macrophages and interstitial cells of the proximal and distal portions of the alveolar duct following crystalline silica exposure. There was less CuZn-SOD staining in epithelial cells at terminal bronchioles in the crystalline silica-exposed group. These findings suggest that these SOD isozymes may be related to lung injury induced by crystalline silica.
关键词：Extracellular superoxide dismutase;Manganese-containing superoxide dismutase;Copper- and zinc-containing superoxide dismutase;Reactive oxygen species;Crystalline silica