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标题：Expression of Heme Oxygenase-1 in the Lungs of Rats Exposed to Crystalline Silica
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作者：Hiroko Nagatomo ; Yasuo Morimoto ; Takako Oyabu 等
期刊名称：Journal of Occupational Health
印刷版ISSN：1341-9145
电子版ISSN：1348-9585
出版年度：2006
卷号：48
期号：2
页码：124-128
DOI：10.1539/joh.48.124
出版社：Japan Society for Occupational Health
摘要：Oxidative stress is thought to be the pathogenesis of pulmonary fibrosis induced by particles, and heme oxygenase-1 (HO-1) protects lung tissue against oxidative stress. We hypothesized that HO-1 is also associated with oxidative lung injury caused by exposure to particles. The present study was conducted to investigate the time course of HO-1 expression of lungs exposed to crystalline silica in vivo . Male Wistar rats were administered 1 mg or 2 mg of crystalline silica suspended in saline by a single intratracheal instillation and were sacrificed at 3 d, 1 wk, 1 month, 3 months and 6 months of recovery time. The expression of HO-1 was observed by western blot analysis and immunostaining. Protein levels of HO-1 were increased compared to the controls at 3 d, and from 1 month to 6 months following intratracheal instillation of 2 mg of crystalline silica. The levels of HO-1 were increased compared to the controls from 1 month to 6 months following intratracheal instillation of 1 mg of crystalline silica. Many HO-1 positive cells were found particularly in the alveolar macrophages during immunostaining. These findings suggest that HO-1 is related to lung injury arising from exposure to crystalline silica.
关键词：Crystalline silica;Heme oxygenase-1;Oxidative stress;Pulmonary fibrosis