文章基本信息

标题：The Effects of 1-Nitropyrene on Oxidative DNA Damage and Expression of DNA Repair Enzymes
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作者：Yong-Dae Kim ; Young-Jun Ko ; Toshihiro Kawamoto 等
期刊名称：Journal of Occupational Health
印刷版ISSN：1341-9145
电子版ISSN：1348-9585
出版年度：2005
卷号：47
期号：3
页码：261-266
DOI：10.1539/joh.47.261
出版社：Japan Society for Occupational Health
摘要：Nitropyrenes (NPs) present in diesel and gasoline emissions are mutagenic and carcinogenic in experimental animals. Nitro-reduction of 1-NP causes oxidative stress. It is unclear whether 8-hydroxydeoxyguanosine (8-OH-dG) is produced from 1-NP and whether it contributes to the carcinogenic activity of 1-NP. In this study, we measured the level of reactive oxygen species (ROS) in cultured human lung epithelial cells after exposure to 1-NP and the intracellular level of 8-OH-dG and expression level of the 8-OH-dG repair enzymes. As results, 1-NP induced the generation of 8-OH-dG via ROS, but 8-OH-dG repair enzymes prevented an increase of 8-OH-dG formation in cellular DNA of the A549 cell line below 250 μ M of 1-NP. These data suggest that 1-NP can induce oxidative DNA damage by generation of ROS, which may play a role in the carcinogenesis induced by 1-NP. These data also suggest that individuals with impaired DNA repair enzymes might be more susceptible to lung cancer induced by 1-NP.
关键词：1-Nitropyrene;Oxidative stress;8-Hydroxydeoxyguanosine;Repair enzymes;Lung cancer;DNA damage