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标题：High Dose of Pyridoxine Induces IGFBP-3 mRNA Expression in MCF-7 Cells and Its Induction Is Inhibited by the p53-Specific Inhibitor Pifithrin-α
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作者：Miwa NAKARI ; Hiroaki KANOUCHI ; Tatsuzo OKA 等
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：2011
卷号：57
期号：4
页码：280-284
DOI：10.3177/jnsv.57.280
出版社：Center for Academic Publications Japan
摘要：It has been reported that supplementation with high-dose vitamin B₆ (B₆) exerts antitumor effects in rodent models of cancer. However, the mechanism of these effects remains poorly understood. High-dose B₆ also suppresses cell proliferation and induces apoptosis of human breast adenocarcinoma MCF-7 cells. Based on preliminary experiments using DNA microarray analyses, we hypothesized that high-dose pyridoxine (PN) might induce IGF-binding protein-3 (IGFBP-3) expression in MCF-7 cells. In this study, we investigated IGFBP-3 induction by 3 or 10 m M PN using a quantitative real-time PCR method. We found that the induction reached a maximum of 24-fold with 10 m M PN for 72 h compared with non-treated cells. The induction of IGFBP-3 by PN was inhibited by a p53-specific inhibitor, pifithrin-α, in a dose-dependent manner, but was not affected by PD169316 (MAPK inhibitor), AS601245 (c-Jun N-terminal kinase inhibitor) or SL327 (MEK1/2 inhibitor). High-dose PN did not induce p53 mRNA expression. The IGFBP-3 induction by PN seemed to be related to p53 activation.
关键词：vitamin B6;pyridoxine;MCF-7;IGFBP-3;p53