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标题：Utilization of Orally Administered D-[14C]Mannitol via Fermentation by Intestinal Microbes in Rats
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作者：Ryoko HONGO ; Sadako NAKAMURA ; Tsuneyuki OKU 等
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：2010
卷号：56
期号：6
页码：387-395
DOI：10.3177/jnsv.56.387
出版社：Center for Academic Publications Japan
摘要：To investigate the available energy of orally administered [¹⁴C]mannitol via intestinal microbes, [¹⁴C]mannitol (222 kBq, 105 mg) or [¹⁴C]glucose (222 kBq, 105 mg) was administered to conventional rats and antibiotics-treated rats whose intestinal microbes were depleted by drinking water containing antibiotics, respectively. The exhausted CO₂, feces and urine were then separately collected at 2, 4, 6, 8, 10, 12 and 24 h after administration of the test solution. In the conventional rats, 45% of administered radioactivity was recovered as ¹⁴CO₂ in the administration of [¹⁴C]mannitol, while 57% of administered radioactivity was recovered as ¹⁴CO₂following the administration of [¹⁴C]glucose for 24 h. The time sequence for the ¹⁴CO₂ excretion from [¹⁴C]mannitol was delayed as compared to [¹⁴C]glucose by about 4-6 h ( p <0.05). However, when [¹⁴C]mannitol was orally administered to antibiotics-treated rats, only 3% of administered radioactivity was excreted as ¹⁴CO₂for 24 h. The total radioactivity of the gastrointestinal contents and feces for 24 h after administration was over 70%, much higher than those of the conventional rats ( p <0.05). When a half dose (222 kBq, 52.5 mg) of [¹⁴C]mannitol was administered to conventional rats, the recovery as ¹⁴CO₂ for 24 h (%) was significantly higher than that of a regular dose of [¹⁴C]mannitol (105 mg). When cold mannitol (105 mg) was orally administered to the antibiotics-treated rats, about 9% of intact mannitol was excreted in feces within 48 h after administration. However, no intact mannitol was detected in the conventional rats. These results demonstrate that more than 95% of mannitol administered orally is utilized via fermentation by intestinal microbes.
关键词：mannitol;antibiotics;intestinal microbes;fermentation;rats