文章基本信息

标题：Protective Effect of Lipoic Acid against Acrolein-Induced Cytotoxicity in IMR-90 Human Fibroblasts
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作者：Lihong JIA ; Zhiyu ZHANG ; Lingling ZHAI 等
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：2009
卷号：55
期号：2
页码：126-130
DOI：10.3177/jnsv.55.126
出版社：Center for Academic Publications Japan
摘要：Acrolein is a highly reactive unsaturated hazardous air pollutant of human health concern, particularly as a component of cigarette smoke. In this study, the effects of acrolein on mitochondrial damage in IMR-90 (a human lung fibroblast cell line), and the reduction of this damage by R- α-lipoic acid were examined. Our results show that acute acrolein exposure exceeding 100 μM (24 h) in IMR-90 cells caused serious cytotoxicity, including decreases in cell viability, mitochondrial membrane potential, SOD activity, GSH and ATP levels, and acute exposure also increased in ROS levels. Pretreatment with R- α-lipoic acid effectively protected IMR-90 cells from acrolein toxicity. The results show that acrolein is a mitochondrial toxin in IMR-90 cells and that acrolein-induced oxidative mitochondrial dysfunction is reduced by R- α-lipoic acid. These experiments imply R- α-lipoic acid may be an effective antioxidant for reducing or preventing chronic oxidant-induced lung cells degeneration in vivo from a variety of sources, including cigarette smoke.
关键词：lipoic acid;acrolein;cytotoxicity;oxidative stress