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标题：Transport of High Concentration of Thiamin, Riboflavin and Pyridoxine across Intestinal Epithelial Cells Caco-2
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作者：Magdalena ZIELINSKA-DAWIDZIAK ; Katarzyna GRAJEK ; Anna OLEJNIK 等
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：2008
卷号：54
期号：6
页码：423-429
DOI：10.3177/jnsv.54.423
出版社：Center for Academic Publications Japan
摘要：In this study, we examined the intestinal uptake of thiamin (vitamin B₁), riboflavin (vitamin B₂) and pyridoxine (vitamin B₆) administered at high concentration using intestinal epithelial Caco-2 cells as an in vitro model of drugs and food absorption. The effect of vitamin concentration, culture age, transport direction and incubation temperature on vitamin transport was determined. The vitamin transport was expressed as an apparent permeability coefficient and changes in cumulative fraction transported across epithelial membrane in time. It was found that transepithelial transport of these vitamins is dependent on the experimental factors. At low concentrations an active transport mechanism was observed, whereas at high vitamin concentration a passive transport dominated. At high vitamin concentration the transepithelial flux of vitamins in both directions was similar, which proves the mechanism of passive transport.
关键词：Caco-2;thiamin;pyridoxine;riboflavin;transport