文章基本信息

标题：Protective Effect of Lipoic Acid against Methylglyoxal-Induced Oxidative Stress in LLC-PK1 Cells
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作者：Hyun Young KIM ; Yasuyuki OI ; Mujo KIM 等
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：2008
卷号：54
期号：1
页码：99-104
DOI：10.3177/jnsv.54.99
出版社：Center for Academic Publications Japan
摘要：Methylglyoxal (MG), a reactive dicarbonyl compound, is a metabolic byproduct of glycolysis often found at high levels in blood from diabetic patients. The effect of lipoic acid on MG-induced oxidative stress was investigated using LLC-PK₁ renal tubular epithelial cells, which are susceptible to oxidative stress. MG (500 μ M ) treatment induced LLC-PK₁ cell death to nearly 50% compared with non-treated control cells, but lipoic acid significantly inhibited the MG-induced cytotoxicity in a concentration-dependent manner. In addition, lipoic acid treatment dose-dependently reduced the intracellular reactive oxygen species level increased by 500 μ M MG. The nitric oxide level was also increased by 500 μ M MG treatment, but it was significantly inhibited by lipoic acid. Furthermore, lipoic acid treatment at 50 μ M inhibited the nuclear translocation of nuclear factor-kappa B induced by MG treatment in LLC-PK₁ cells. These findings indicate that lipoic acid has potential as a therapeutic agent against the development of diabetic complications related to MG-induced oxidative stress in diabetes.
关键词：lipoic acid;oxidative stress;reactive oxygen species;NO;NF-κB