文章基本信息

标题：Rapid and Convenient Detection of Urinary Equol by Thin-Layer Chromatography
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作者：Shin-ichiro YOKOYAMA ; Tsuyoshi KUZUGUCHI
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：2007
卷号：53
期号：1
页码：43-47
DOI：10.3177/jnsv.53.43
出版社：Center for Academic Publications Japan
摘要：Equol, a metabolite of daidzein for some intestinal microflora, is known to retain highly estrogenic activity and is of wide interest in relation to human health. However, not all humans can produce equol. In this study, detection of urinary equol using thin-layer chromatography (TLC) was performed to distinguish between equol producers and non-producers. After 36 h of soy food intake, urine, collected from 7 volunteers, was hydrolyzed, purified by reverse phase silica gel column and applied to normal phase TLC. Consequently, equol was clearly separated from the urine samples and discriminated the equol producers in this system. The detection limit of equol was at least 20 ng.
关键词：equol;detection;urine;thin-layer chromatography