文章基本信息

标题：PGC-1α in the myofibers regulates the balance between myogenic and adipogenic progenitors affecting muscle regeneration
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作者：Marc Beltrà ; Fabrizio Pin ; Domiziana Costamagna 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2022
卷号：25
期号：11
页码：1-19
DOI：10.1016/j.isci.2022.105480
语种：English
出版社：Elsevier
摘要：SummarySkeletal muscle repair is accomplished by satellite cells (MuSCs) in cooperation with interstitial stromal cells (ISCs), but the relationship between the function of these cells and the metabolic state of myofibers remains unclear. This study reports an altered proportion of MuSCs and ISCs (including adipogenesis-regulatory cells; Aregs) induced by the transgenic overexpression of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) in the myofibers (MCK-PGC-1α mice). Although PGC-1α–driven increase of MuSCs does not accelerate muscle regeneration, myogenic progenitors isolated from MCK-PGC-1α mice and transplanted into intact and regenerating muscles are more prone to fuse with recipient myofibers than those derived from wild-type donors. Moreover, both young and aged MCK-PGC-1α animals exhibit reduced perilipin-positive areas when challenged with an adipogenic stimulus, demonstrating low propensity to accumulate adipocytes within the muscle. Overall, these results unveil that increased PGC-1α expression in the myofibers favors pro-myogenic and anti-adipogenic cell populations in the skeletal muscle.Graphical abstractDisplay OmittedHighlights•Myofiber-specific PGC-1α overexpression alters the proportion of MuSCs and ISCs•Injection of MCK-PGC-1α cells promotes oxidative conversion of WT myofibers•MCK-PGC-1α mice are protected from intramuscular adipocyte accumulationPhysiology; Cell biology; Developmental biology