文章基本信息

标题：写真ゼラチンのSDS電気泳動若干の基礎的・定性的検討
本地全文：下载
作者：青柳象平 ; 平柳公子 ; 松本年雄等
期刊名称：日本写真学会誌
印刷版ISSN：0369-5662
电子版ISSN：1884-5932
出版年度：1987
卷号：50
期号：1
页码：14-21
出版社：The Society of Photographic Science and Technolgy of Japan
摘要：For improving the accuracy of molecular weight distribution (MWD) analysis of photographic gelatin, electrophoretic conditions in sodium dodecyl sulfate/polyacrylamide gel system (SDS/PAGE) were investigated in detail.A good separation was obtained by overlaying a concentrating layer on the separating gel with a gradient of 2.5 to 10% acrylamide.The result of various pretreatments strongly suggests that dodecyl sulfate ions rapidly bind to gelatin molecules at room temperature and also that the resulted complexes directly reach the most stable conformation without staying at metastable states.From the data of 33 IAG gelatins, some characteristics of MWD patterns in the gelatin types have been recognized.The result of silver staining suggests the existence of certain selective interactions between gelatin molecules and silver ions.