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标题：Avaliação de antígenos através do ensaio imunoenzimático ELISA ao longo de infecção experimental em caprinos com Corynebacterium pseudotuberculosis
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作者：Victoria Christiane Wense Gazarian Calderon ; José Tadeu Raynal Rocha Filho ; Maria Conceição Aquino de Sá 等
期刊名称：Research, Society and Development
电子版ISSN：2525-3409
出版年度：2022
卷号：11
期号：12
页码：1-14
DOI：10.33448/rsd-v11i12.34549
语种：English
出版社：Grupo de Pesquisa Metodologias em Ensino e Aprendizagem em Ciências
摘要：Corynebacterium pseudotuberculosis is the etiologic agent of caseous lymphadenitis (CL), a chronic infectious disease that affects sheep and goats. Numerous serological tests have been developed to detect the disease, being the Enzyme Immunoassay (ELISA) one of the most used due to its advantages of sensitivity and specificity. The present study aimed to evaluate the reactivity of a panel of Corynebacterium pseudotuberculosis antigens in goats experimentally infected with non-forming and biofilm-forming strains. Eighteen Canindé goats were used: group 1 - negative control animals, group 2 - goats infected with non-biofilm-forming bacteria and group 3 - goats infected with a biofilm-forming strain. Recombinant antigens CP40 and PLD, membrane fraction antigens (F3) and C. pseudotuberculosis secreted antigens (BHI) were tested. The sensitivity and specificity of the indirect ELISA tests for all antigens were 100%. However, the optical density (OD) values recorded in the BHI ELISA were lower than those found in the other ELISA tests, suggesting that specific antigens may have better sensitivity and specificity in the diagnosis of CL.
关键词：Corynebacterium pseudotuberculosis;Immunodiagnosis;Caseous lymphadenitis;Goats.