文章基本信息

标题：Primeiro relato de produção de colagenase por fungo isolado do solo da Caatinga - Rhizopus microsporus UCP 1296
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作者：Isabele Albuquerque Alcoforado Ferreira ; Maria Carolina de Albuquerque Wanderley ; Ana Lucia Figueiredo Porto 等
期刊名称：Research, Society and Development
电子版ISSN：2525-3409
出版年度：2022
卷号：11
期号：12
页码：1-11
DOI：10.33448/rsd-v11i12.34618
语种：English
出版社：Grupo de Pesquisa Metodologias em Ensino e Aprendizagem em Ciências
摘要：The present work aimed to study the fungus Rhizopus microsporus UCP 1296 isolated from the Caatinga soil, emphasizing the development and application of biotechnological processes for the production of a new collagenase. Therefore, significant fermentation parameters such as temperature, pH, culture medium and fermentation time were evaluated. Samples of the microorganism were kept at 4 °C in malt extract agar medium. The mycelium was suspended in 5 mL of a solution composed of NaCl and Tween 80. After inoculation with the spore suspension containing 150 µL (106 spores/mL), submerged fermentation was carried out in Erlenmeyer flasks containing 250 mL of gelatin. A growth curve was developed to identify the best time for enzyme production. The results show that collagenase production occurred with 120 hours of fermentation, obtaining 550 U/mL of collagenolytic activity and 0.42 g/L biomass in the stationary phase, indicating that the collagenase produced is a secondary metabolite. A 24 factorial design was carried out to select the best parameters where the influence of the variables was evaluated: temperature (ºC), pH, agitation and substrate concentration (gelatin). As a result of the factorial design, we obtained an increase in enzyme production of about 32%. This is the first study to describe optimized conditions for collagenase production by R. microsporus strains.
关键词：Collagenase;Filamentous fungus;Bioprocesses;Innovation.