文章基本信息

标题：Transcriptomic Response of Human Nosocomial Pathogen Pseudomonas aeruginosa Biofilms Following Continuous Exposure to Antibiotic-Impregnated Catheters
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作者：Kidon Sung ; Dan Li ; Jungwhan Chon 等
期刊名称：Data
印刷版ISSN：2306-5729
出版年度：2022
卷号：7
期号：3
页码：1-7
DOI：10.3390/data7030035
语种：English
出版社：MDPI Publishing
摘要：Biofilms are complex surface-attached bacterial communities that serve as a protectivesurvival strategy to adapt to an environment. Bacterial contamination and biofilm formation onimplantable medical devices pose a serious threat to human health, and these biofilms have becomethe most important source of nosocomial infections. Although antimicrobial-impregnated cathetershave been employed to prevent bacterial infection, there have been concerns about the potentialemergence of antibiotic resistance. To investigate the risk of developing resistance, we performedRNA-sequencing gene expression profiling of P. aeruginosa biofilms in response to chronic exposure toclindamycin and rifampicin eluted from antibiotic-coated catheters in a CDC biofilm bioreactor. Therewere 877 and 178 differentially expressed genes identified in planktonic and biofilm cells after growthfor 144 h with control (without antibiotic-impregnation) and clindamycin/rifampicin-impregnatedcatheters, respectively. The differentially expressed genes were further analyzed by Clusters ofOrthologous Groups (COGs) functional classification and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway analyses. The data are publicly available through the GEO database with accessionnumber GSE153546.
关键词：RNA-seq;P. aeruginosa PAO1;clindamycin/rifampicin-impregnated;catheters