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标题：Aged microplastics change the toxicological mechanism of roxithromycin on Carassius auratus: Size-dependent interaction and potential long-term effects
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作者：Peng Zhang ; Guanghua Lu ; Yu Sun 等
期刊名称：Environment International
印刷版ISSN：0160-4120
电子版ISSN：1873-6750
出版年度：2022
卷号：169
页码：1-13
DOI：10.1016/j.envint.2022.107540
语种：English
出版社：Pergamon
摘要：Graphical abstractDisplay OmittedHighlights•AMPs promoted the bioaccumulation of ROX in tissues with size dependence.•Smaller AMPs exacerbated ROX-induced pathological abnormalities in liver and brain.•Larger AMPs caused more severe intestinal damage and higher pathogen abundance.•Long-term effects of AMPs and ROX on microbial metabolism were size-dependent.AbstractSize effects of microplastics have received extensive attention for their influence on other pollutants and harm to organisms. In this study, we investigated the uptake, elimination, tissue distribution and potential toxicity mechanism of roxithromycin (ROX) in the presence of 0.5, 5 and 50 μm of aged microplastics (AMPs) inCarassius auratus. The results showed that AMPs promoted the ROX bioaccumulation of various tissues in a size-dependent manner. AMPs and ROX significantly induced superoxide dismutase and catalase activities of liver and gut, and inhibited acetylcholinesterase activities of brain. The coexistence of smaller AMPs exacerbated pathological abnormalities in liver, gill and brain induced by ROX, while larger AMPs caused more intestinal damage. Moreover, high-throughput 16S rRNA gene sequencing indicated that the abundance ofProteobacteriain 0.5 μm AMPs and ROX joint treatments andFirmicutesandBacteroidotain 50 μm AMPs and ROX joint treatments were significantly raised (p < 0.05). Metabolomics revealed that AMPs and ROX had a size-dependent long-term effect on gut microbial metabolites, which was mainly related to galactose metabolism, amino acid metabolism and primary bile acid biosynthesis pathways after a 7-day elimination, respectively. These results provide important insights into the relationship between the size effect of AMPs and interaction mechanism of AMPs and coexisting pollutants on aquatic organisms.
关键词：KeywordsAged microplasticsGut microbiotaToxicological mechanismSize-dependent interactionLong-term effects