文章基本信息

标题：Cellular level of coenzyme Q increases with neuronal differentiation, playing an important role in neural elongations
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作者：Akari Nakamura ; Mizuho Okamoto ; Ayaka Maeda 等
期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：2022
卷号：71
期号：2
页码：89-96
DOI：10.3164/jcbn.21-107
语种：English
出版社：The Society for Free Radical Research Japan
摘要：Deficiency of coenzyme Q has been reported in various neurological diseases, and the behavior of this lipid in neurons has attracted attention. However, the behavior of this lipid in normal neurons remains unclear. In this study, we analyzed the concentration of coenzyme Q before and after neuronal differentiation. Nerve growth factor treatment of PC12 cells caused neurite outgrowth and neuronal differentiation, and the amount of intracellular coenzyme Q increased dramatically during this process. In addition, when the serum was removed from the culture medium of N1E-115 cells and the neurite outgrowth was confirmed, the intracellular coenzyme Q level also increased. To elucidate the role of the increased coenzyme Q, we administered nerve growth factor to PC12 cells with coenzyme Q synthesis inhibitors and found that coenzyme Q levels decreased, neurite outgrowth was impaired, and differentiation markers were reduced. These results indicate that coenzyme Q levels increase during neuronal differentiation and that this increase is important for neurite outgrowth.
关键词：coenzyme Q;neural differentiation;neurite outgrowth;NGF;PC12 cells