文章基本信息

标题：葉緑体SSR領域を用いたイネ科植物のDNA多型解析
本地全文：下载
作者：荒木直幸 ; 吉川ひとみ ; 柘浩一郎等
期刊名称：日本法科学技術学会誌
印刷版ISSN：1880-1323
电子版ISSN：1881-4689
出版年度：2022
卷号：27
期号：2
页码：211-219
DOI：10.3408/jafst.826
语种：Japanese
出版社：Japanese Association of Forensic Science and Technology
摘要：Identification of unknown plant samples collected from crime scenes is carried out by morphological observation or plant DNA barcoding. However, DNA barcoding is rarely performed because the DNA typing performed by forensic science laboratories in Japanese police departments generally does not involve DNA sequencing techniques. Therefore, it is necessary to develop plant identification and discrimination methods involving DNA fragment analysis that can be performed in these laboratories. A total of 66 plant samples (Poaceae, Typhaceae, and Cyperaceae) were analyzed by examining 8 sets of consensus primer pairs in simple sequence repeat regions of the chloroplast genome. The DNA fragment lengths of the PCR amplification products were compared and classified into 61 types. The results of this study may aid in the classification and identification of unknown plant samples.
关键词：Simple sequence repeat region;Chloroplast DNA polymorphism;Poaceae