文章基本信息

标题：Exogenous S-adenosyl-L-methionine Could Inhibit c-myc Overexpression Induced by As2O3 in Normal Human Liver HL-7702 Cells
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作者：Yongmei Qi ; Ailing Li ; Dejun Huang 等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：2011
卷号：57
期号：2
页码：188-191
DOI：10.1248/jhs.57.188
出版社：The Pharmaceutical Society of Japan
摘要：Arsenic trioxide (As₂O₃) is known to promote liver and other cancers due to its ability to decrease the level of S-adenosyl-L-methionine (SAM), thus leading to the abnormal expression of oncogene c- myc . The present study investigated the effect of exogenous SAM on As₂O₃-induced c- myc expression in the normal human liver cells, HL-7702. c- myc expression increased approximately 50% after As₂O₃ treatments (0-20 μM) for 24 hr. However, this elevated c- myc expression is significantly inhibited by 0.2 μM SAM. Co-treatment of HL-7702 cells with 1.0 μM As₂O₃ and 0.2 μM SAM for 24 and 30 hr, caused prominent inhibitory effects on c- myc overexpression. The results indicate SAM could inhibit c- myc overexpression induced by As₂O₃ in HL-7702 cells. These findings may be of significance to the exploration of SAM in preventing arsenic carcinogenesis.
关键词：arsenic trioxide;S-adenosyl-L-methionine;c-myc;reverse transcription-polymerase chain reaction