文章基本信息

标题：Geniposide Prevents PC12 Cells from Peroxynitrite via the Mitogen-Activated Protein Kinase Signaling Pathway
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作者：Fei Yin ; Jianhui Liu ; He Xiao 等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：2010
卷号：56
期号：2
页码：195-199
DOI：10.1248/jhs.56.195
出版社：The Pharmaceutical Society of Japan
摘要：Reactive oxygen species play an important role in the development of neurodegenerative diseases, including Alzheimer's disease (AD), Parkinson's disease (PD) and stroke. The present study investigated the neuroprotective action of geniposide and its signaling pathway in peroxynitrite induced PC12 cells. After coculture of PC12 cells with geniposide in the presence or absence of 3-morpholinosydnonmine hydrochloride (SIN-1), a donor of peroxynitrite, Bcl-2, Bcl-2 associated X protein (Bax) and the phosphorylation of mitogen-activated protein kinase (MAPK) were probed with Western blotting. The results showed that geniposide enhanced the expression of Bcl-2 to prevent the peroxynitrite damage induced by SIN-1 in PC12 cells. U0126, a selective inhibitor of MAPK/ERK, attenuated the protective effects of geniposide by inhibiting the phosphorylation of MAPK and expression of Bcl-2. All these data indicate that geniposide prevents PC12 cells from peroxynitrite damage induced by SIN-1 involved in the MAPK signaling pathway.
关键词：geniposide;peroxynitrite;Bcl-2;mitogen-activated protein kinase