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标题：Impact of Pueraria candollei Root Cultures on Cytochrome P450 2B9 Enzyme and Lipid Peroxidation in Mice
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作者：Latiporn Udomsuk ; Kanokporn Churikawit ; Waraporn Putalun 等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：2010
卷号：56
期号：2
页码：182-187
DOI：10.1248/jhs.56.182
出版社：The Pharmaceutical Society of Japan
摘要：Pueraria candollei (Leguminosae) has long been used as a traditional medicine for rejuvenation, and the major constituents in the tuberous root are isoflavonoids. The effects of the root cultures of P. candollei (r-PC) on cytochrome P450 enzymes in mouse liver and antioxidant activity in mouse brain including gross anatomy were examined. r-PC significantly enlarged the uterus length while no change in weight was noted. The total P450 content was not significantly altered by either estradiol benzoate (ES) or r-PC. Benzyloxyresorufin O -dealkylase activity was significantly increased by r-PC, comparable to the increase induced by ES. Correspondingly, r-PC demonstrated a tendency to upregulate CYP2B9 mRNA expression. Furthermore, assessment of lipid peroxidation in mouse brain using the thiobarbituric acid assay demonstrated that r-PC remarkably decreased the level of malondealdehyde formation. These observations suggest r-PC as a potential alternative to ES with the extrabenefit of its antioxidant activity.
关键词：Pueraria candollei;benzyloxyresorufin O-dealkylayion;CYP2B9;estradiol;lipid peroxidation;TBARS