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标题：Roles Played by MerE and MerT in the Transport of Inorganic and Organic Mercury Compounds in Gram-negative Bacteria
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作者：Yuka Sone ; Hidemitsu Pan-Hou ; Ryosuke Nakamura 等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：2010
卷号：56
期号：1
页码：123-127
DOI：10.1248/jhs.56.123
出版社：The Pharmaceutical Society of Japan
摘要：In order to clarify the physiological roles played by MerP, MerT and MerE in Gram-negative bacteria, we constructed the plasmids pTP4 and pTPE21, which contained the genes merR , merT and merP , from the Pseudomonas K-62 plasmid pMR26, or the same genes with the merE gene of Tn 21 from the Shigella flexneri plasmid NR1 (R100), respectively. Cells containing pTP4 showed increased hypersensitivity to Hg(II), but maintained a normal sensitivity to CH₃Hg(I). However, cells with pTPE21 exhibited increased hypersensitivity to Hg(II) and CH₃Hg(I). Cells with pTP4 accumulated appreciably more Hg(II) than control cells, but no significant difference was observed in their uptake of ¹⁴CH₃Hg(I). In contrast, the cells containing pTPE21 accumulated significantly larger amounts of Hg(II) and ¹⁴CH₃Hg(I) than either control cells or cells with pTP4. These results suggest that the mer operons have evolved to redirect uptake of mercurials into dedicated, specific and relatively high-affinity transport systems comprising the small periplasmic protein MerP and two inner membrane proteins, MerT and MerE.
关键词：merE;merT;merP;methylmercury transport;broad-type mercurial transporter