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  • 标题:Distribution and Diversity of Shiga Toxin 2 Gene in Urban Rivers
  • 作者:Katsuji Tani ; Misuzu Kaneshige ; Masao Nasu
  • 期刊名称:Journal of Health Science
  • 印刷版ISSN:1344-9702
  • 电子版ISSN:1347-5207
  • 出版年度:2007
  • 卷号:53
  • 期号:4
  • 页码:486-490
  • DOI:10.1248/jhs.53.486
  • 出版社:The Pharmaceutical Society of Japan
  • 摘要:In urban rivers in Osaka, Japan, DNA containing the stx 2 gene, which encodes the Shiga toxin 2 (Stx2), was found to be present in sediment, even when it was not detected in the surface water. A DNA sequence similar to that of bacteriophage 933W and the Sakai strain was detected at every sampling location. Two strains of Escherichia coli O157 carrying the stx 2 gene were independently isolated from sediment. These results show that river sediment is a potential reservoir of the stx 2 gene and Shiga toxin-producing bacteria in the natural environment.
  • 关键词:Shiga toxin 2 gene;river;distribution;diversity;most probable number-nested polymerase chain reaction
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