摘要:In urban rivers in Osaka, Japan, DNA containing the stx 2 gene, which encodes the Shiga toxin 2 (Stx2), was found to be present in sediment, even when it was not detected in the surface water. A DNA sequence similar to that of bacteriophage 933W and the Sakai strain was detected at every sampling location. Two strains of Escherichia coli O157 carrying the stx 2 gene were independently isolated from sediment. These results show that river sediment is a potential reservoir of the stx 2 gene and Shiga toxin-producing bacteria in the natural environment.