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标题：Differential Effects of Buckwheat and Kudingcha Extract on Neuronal Damage in Cultured Hippocampal Neurons and Spatial Memory Impairment Induced by Scopolamine in an Eight-Arm Radial Maze
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作者：Fengling Pu ; Kenichi Mishima ; Keiichi Irie 等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：2005
卷号：51
期号：6
页码：636-644
DOI：10.1248/jhs.51.636
出版社：The Pharmaceutical Society of Japan
摘要：We have reported the neuroprotection provided by an extract of buckwheat (BWE, Fagopyrum esculentum Moench) on neuronal damage in the repeated cerebral ischemia model and demonstrated that BWE inhibited the excess glutamate release induced by repeated cerebral ischemia, suggesting that BWE had a free radical-scavenging in addition to anti-glutamate action. In the present study, we studied the neuroprotective effects of Kudingcha extract (KDE, Ligustrum purpurascens Y. C.) on scavenging by the 2,2-diphenyl-1-picylhydrazyl (DPPH) radical in primary cultured hippocampal neurons, and on spatial memory impairment induced by scopolamine in an eight-arm radial maze in comparison with BWE. The effects of KDE (0.01-1 mg/ml) were more potent than those of BWE (0.01-1 mg/ml) in scavenging the DPPH radical (1 mM). KDE (100 μ g/ml) prevented the cell damage induced by glutamate (300 μ M) or kainate (1 mM), which was more potent than BWE (100 μ g/ml), but BWE suppressed the cellular damage induced by β -amyloid_(25-35) (20 μ M) more potently than KDE (100 μ g/ml). BWE (600 mg/kg), but not KDE, significantly suppressed the increase in errors induced by scopolamine (0.5 mg/kg i.p.) in the eight-arm radial maze. The results suggest that BWE may protect against cholinergic dysfunction and that KDE protects more effectively against glutaminergic dysfunction.
关键词：buckwheat extract;Kudingcha extract;2,2-diphenyl-1-picylhydrazyl radical;primary cultured hippocampal neuron;scopolamine