摘要：We investigated the estrogenic activity of a bisphenol A (BPA) metabolite (4-methyl-2,4-bis( p -hydroxyphenyl)-pent-1-ene; MBP) in male medaka ( Oryzias latipes ) using vitellogenin (Vg, Vg1 and Vg2) as a biomarker. Male d-rR medaka were exposed to various concentrations of estradiol-17 β (E2), MBP and BPA for 3 days, and then the serum Vg concentration was measured using specific chemiluminescent immunoassays. The estimated relative estrogenic activities of MBP and BPA compared with E2 (100%) were 1.3-1.4% and 0.00010-0.00023%, respectively. These findings indicated that MBP has about 104-fold higher estrogenic potency than the parent BPA and about 1/50 that of E2 for Vg synthesis in medaka. This is the first study to show that MBP can act as a highly potent estrogen agonist in living organisms.
关键词：bisphenol A metabolite;estrogenic activity;vitellogenin;4-methyl-2,4-bis(p-hydroxyphenyl)-pent-1-ene;medaka
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