标题：17β-Estradiol Primes Elicitation of Inducible Nitric Oxide Synthase Expression by Lipopolysaccharide and Interferon-γ in Mouse Macrophage Cell Line J774.1
摘要：The effects of estrogenic compounds on nitric oxide (NO) production by macrophages were examined. 17 β -Estradiol promoted NO production triggered by lipopolysaccharide (LPS) and/or interferon (IFN)- γ in the mouse macrophage cell line J774.1. Other estrogen-like substances such as estrone, 17 α -ethynylestradiol and bisphenol A also enhanced NO synthesis, but this NO synthesis was not activated by Ca2+ ionophore A23187. RT-PCR analysis demonstrated induction of inducible nitric oxide synthase (iNOS) mRNA in J774.1 cells exposed to 17 β -estradiol. Although the estrogen receptor (ER)-antagonist ICI-182780 partially suppressed the promoting effect of 17 β -estradiol on NOS activity, there was little ER α detectable by RT-PCR from J774.1 cells. These results suggest that ERs may participate only partially in iNOS mRNA transcription in J774.1 cells and that 17 β -estradiol may act directly through other unknown intracellular signal transduction(s) that are activated by LPS and IFN- γ .
关键词：17β-estradiol;macrophage;nitric oxide;endocrine disrupting chemical
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