文章基本信息

标题：1-Chloro-2,4-Dinitrobenzene Stimulates the Estrogenic Activity in MCF-7 Cells
作者：Joohee Jung ; Kunie Ishida ; Shigehiro Osada 等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：2004
卷号：50
期号：6
页码：581-587
DOI：10.1248/jhs.50.581
出版社：The Pharmaceutical Society of Japan
摘要：The estrogenic effect of chemicals, including 1-chloro-2,4-dinitrobenzene (CDNB), in the combination with 17 β -estradiol (E₂) was screened by a reporter gene assay using breast cancer cells (MCF-7). It was found that CDNB stimulated E₂-induced transcriptional activity of estrogen receptor (ER) and down-regulated both ER protein and mRNA. However, CDNB alone and CDNB metabolite(s) showed no estrogenic activity and no binding activity to ER, suggesting an indirect pathway other than ER. CDNB gave no transcriptional activity for an aryl hydrocarbon receptor (AhR), suggesting no possibility of a pathway through cross-talk between AhR and ER. On the other hand, CDNB enhanced the mitogen activated protein kinase (MAPK) pathway, suggesting estrogenic action via MAPK. These results indicated that CDNB possessed estrogen-like activity in the transcription and regulation of ER though a different pathway from E₂.
关键词：1-chloro-2,4-dinitrobenzene;metabolite;aryl hydrocarbon receptor;mitogen activated protein kinase;MCF-7 cell