摘要：The deacylation of N -formylanilines and N -acetylanilines to aniline derivatives was examined in rat liver preparations. When N 4-formylsulfanilamide or N 4-acetylsulfanilamide was incubated with rat liver cytosol, the deacylated metabolite, sulfanilamide, was formed. These deacylating activities were inhibited by paraoxone and diisopropyl fluorophosphate, inhibitors of formamidase. N 4-Formylsulfanilamide and N 4-acetylsulfanilamide were deacylated by formamidase purified from rat liver cytosol. N -Acetyl- or N -formylanilines bearing an electron-withdrawing group at the para position were deacylated to anilines by formamidase. In contrast, anilines bearing an electron-donating group at the para position were formylated to N -formylanilines in the presence of N -formyl-L-kynurenine. Formamidase catalyzed both N -formylation of aniline derivatives, and deacylation of N -formylanilines and N -acetylanilines.
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