文章基本信息

标题：Metabolic profilings of rat INS-1 β-cells under changing levels of essential amino acids
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作者：Lianbin Xu ; Xueyan Lin ; Xiuli Li 等
期刊名称：Scientific Data
电子版ISSN：2052-4463
出版年度：2022
卷号：9
期号：1
页码：1-5
DOI：10.1038/s41597-022-01436-w
语种：English
出版社：Nature Publishing Group
摘要：Application of mass spectrometry enables the detection of metabolic diferences between organisms with diferent nutritional settings . Divergence in the metabolic fngerprints of rat pancreatic INS-1 β -cells were systematically captured with regard to ten individual essential amino acid (EAA) availability. A high-resolution tandem mass spectrometry system coupled to liquid chromatography produced a horizontal comparison of metabolic proflings of β-cells with individual EAA elevated to 10 mmol/L by turn or removal individual EAA from the medium one by one . Quality control samples were injected at regular intervals throughout the analytical run to monitor and evaluate the stability of the system . The raw data of samples and reference compounds including study protocols have been deposited in the open metabolomics database MetaboLights to enable efcient reuse of the datasets, such as investigating the diference in metabolic process between diverse EAAs as well as screening and verifying potential metabolites afecting insulin secretion and β-cell function .