文章基本信息

标题：Three-dimensional structured illumination microscopy data of mitochondria and lysosomes in cardiomyoblasts under normal and galactose-adapted conditions
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作者：Ida S.Opstad ; Gustav Godtliebsen ; Florian Ströhl 等
期刊名称：Scientific Data
电子版ISSN：2052-4463
出版年度：2022
卷号：9
期号：1
页码：1-7
DOI：10.1038/s41597-022-01207-7
语种：English
出版社：Nature Publishing Group
摘要：This three-dimensional structured illumination microscopy (3DSIM) dataset was generated to highlight the suitability of 3DSIM to investigate mitochondria-derived vesicles (MDVs) in H9c2 cardiomyoblasts in living or fxed cells . MDVs act as a mitochondria quality control mechanism . The cells were stably expressing the tandem-tag eGFP-mCherry-OMP25-TM (outer mitochondrial membrane) which can be used as a sensor for acidity. a part of the dataset is showing correlative imaging of lysosomes labeled using LysoTracker in fxed and living cells . The cells were cultivated in either normal or glucose-deprived medium containing galactose . The resulting 3DSIM data were of high quality and can be used to undertake a variety of studies. Interestingly, many dynamic tubules derived from mitochondria are visible in the 3DSIM videos under both glucose and galactose-adapted growth conditions . As the raw 3DSIM data, optical parameters, and reconstructed 3DSIM images are provided, the data is especially suitable for use in the development of SIM reconstruction algorithms, bioimage analysis methods, and for biological studies of mitochondria.