文章基本信息

标题：The Importance of a 95-kb Virulence Plasmid in the Pathogenicity of Salmonella Abortusequi in Horses
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作者：Toru ANZAI ; Yasushi KUWAMOTO ; Seiji HOBO 等
期刊名称：Journal of Equine Science
印刷版ISSN：1340-3516
电子版ISSN：1347-7501
出版年度：2005
卷号：16
期号：4
页码：111-116
DOI：10.1294/jes.16.111
出版社：Japanese Society of Equine Science
摘要：To demonstrate the participation of the 95-kb virulence plasmid in the pathogenicity of S. Abortusequi in horses, the prevalence of the plasmid in S. Abortusequi field isolates was examined, and experimental inoculation of mice and horses was carried out using strains with and without the plasmid. Single, large plasmids were detected in 226 of the 242 field isolates (93.4%), and spvC was detected by PCR testing from all the isolates with the plasmid. Mouse virulence tests revealed that the LD₅₀ of 11 of 13 isolates harboring the virulence plasmid was less than 10⁴ CFU, whereas the LD₅₀ of 12 isolates not harboring the virulence plasmid was, without exception, over 10⁶ CFU. Experimentally, 6 horses inoculated with S. Abortusequi strains 17N1 or Sal-107 harboring the 95-kb virulence plasmid were infected, and showed similar clinical symptoms, even though Sal-107 is avirulent in IVCS mice. On the other hand, the 17N3 plasmid-cured strain did not infect horses. We conclude that the 95-kb virulence plasmid of S. Abortusequi plays a role as a pathogenic factor in mice, but is also important in horses.
关键词：horse;Salmonella Abortusequi;virulence plasmid