文章基本信息

标题：3D bioprinting of nanoparticle-laden hydrogel scaffolds with enhanced antibacterial and imaging properties
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作者：Andrea S. Theus ; Liqun Ning ; Gabriella Kabboul 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2022
卷号：25
期号：9
页码：1-19
DOI：10.1016/j.isci.2022.104947
语种：English
出版社：Elsevier
摘要：SummaryBiomaterial-associated microbial contaminations in biologically conducive three-dimensional (3D) tissue-engineered constructs have significantly limited the clinical applications of scaffold systems. To prevent such infections, antimicrobial biomaterials are rapidly evolving. Yet, the use of such materials in bioprinting-based approaches of scaffold fabrication has not been examined. This study introduces a new generation of bacteriostatic gelatin methacryloyl (GelMA)-based bioinks, incorporated with varying doses of antibacterial superparamagnetic iron oxide nanoparticles (SPIONs). The SPION-laden GelMA scaffolds showed significant resistance against theStaphylococcus aureusgrowth, while providing a contrast in magnetic resonance imaging. We simulated the bacterial contamination of cellular 3D GelMA scaffoldsin vitroand demonstrated the significant effect of functionalized scaffolds in inhibiting bacterial growth, while maintaining cell viability and growth. Together, these results present a new promising class of functionalized bioinks to 3D bioprint tissue-engineered scaffold with markedly enhanced properties for the use in a variety ofin vitroand clinical applications.Graphical abstractDisplay OmittedHighlights•Functionalized bioinks with bacteriostatic properties are developed and thoroughly characterized•The 200 μg/mL group yielded an optimal balance of printed scaffold properties•Incorporating nanoparticle also enabled noninvasive imaging of the bioprinted scaffoldBiomaterials; Nanoparticles; Tissue engineering